I just saw Black Widow. Yelena and Natasha say they don’t have any ovaries because the Red Room surgically removed them. That means no estrogen. But those women have definitely been through puberty. If you don’t have any estrogen or testosterone, your bones get weak like a child’s bones. That’s no good for being a super assassin. And I don’t think the Black Widow is on testosterone injections. She’s not hairy enough for it. So is she taking estradiol pills?
Oh, last I remembered Black Cat had the super soldier serum, didn’t know the windows got them too.
Kinda sorta, they had the Russian version that wasn’t as good. No one ever repeated the kind of success Cap saw, (that I know of)